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precision plus proteintm all blue prestained protein standards  (Bio-Rad)


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    Bio-Rad precision plus proteintm all blue prestained protein standards
    Precision Plus Proteintm All Blue Prestained Protein Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1061 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/precision+plus+proteintm+all+blue+prestained+protein+standards/Precision+Plus+Protein+All+Blue+Prestained+Protein+Standards/pm42025918-96-8-16
    Average 96 stars, based on 1061 article reviews
    precision plus proteintm all blue prestained protein standards - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: Systematic real-time profiling of Salmonella type III effector translocation provides quantitative resolution of the T3SS-1/T3SS-2 secretion dichotomy
    Article Snippet: Protein samples for one-dimensional SDS-PAGE were prepared by the addition of XT sample loading buffer (4×; Bio-Rad, cat. no. 1610791) and XT reducing agent (20×; Bio-Rad, cat. no. 1610792) according to the manufacturer’s instructions. .. Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, cat. no. 1610373) were used as molecular weight markers. .. Proteins were resolved on 4–12% gradient or 12% XT precast Criterion gels (Bio-Rad) using XT-MOPS running buffer (Bio-Rad, cat. no. 1610788) at 150–200 V and transferred to 0.45-μm nitrocellulose membranes for 30 min at 100 V using Tris–borate transfer buffer (50 mM Tris (base) and 50 mM boric acid).

    Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components
    Article Snippet: The buffer consisted of NuPAGETM LDS Sample Buffer and dithiothreitol (DTT) at a 9:1 ratio and was denatured by heating at 65°C for 15 minutes prior to loading onto NuPAGETM Bis-Tris 4–12% Midi Protein Gels or NuPAGETM 10% Bis-Tris Gels (Invitrogen). .. Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad) or PageRulerTM Prestained Protein Ladder (Invitrogen) were used as molecular weight markers. .. Electrophoresis was performed using NuPAGETM MOPS SDS Running Buffer (Invitrogen) in a standard electrophoresis chamber.

    Software:

    Article Title: Targeting mitochondrial bioenergetics: the “Achilles’ heel” of Leishmania
    Article Snippet: All chemicals were of analytical grade and obtained from Sigma-Aldrich (St. Louis, MO, USA), except the following: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2 H -tetrazolium (MTS; Promega; Madison, WI, USA); phenazine methosulfate (PMS; Sisco Research Laboratories; Andheri, Mumbai, India); cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA); TRIzol reagent (Ambion, Austin, TX, USA); ThiolTrackerTM Violet, adenosine triphosphatase (ATP) determination kit, and 3,8-phenanthridinediamine,5-(6-triphenylphosphoniumhexyl)-5,6-dihydro-6-phenyl (MitoSOXTM red; Molecular Probes, Carlsbad, CA, USA); fetal bovine serum (FBS, Gibco); SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific, Waltham, MA, USA); Optilux microtest plates for ATP determination and Cell-TakTM (Corning ® , Tewksbury, MA, USA); cell lysis buffer and antibodies against AMPKα (AMP-activated protein kinase-α; CST, cat. no. 5831); mammalian target of rapamycin (mTOR; CST, cat. no. 2983) or their phosphorylated counterpart P-AMPKα (CST, cat. no. 2535); P-mTOR (CST, cat. no. 5536); peroxisome proliferator-activated receptor-gamma coactivator (PGC1α; ABclonal, cat. no. A12348); glucose transporter 1 (GLUT1; ABclonal, cat. no. A6982); β-actin (CST, cat. no. 4970); horseradish peroxidase (HRP)-linked anti-rabbit secondary IgG antibody (ABclonal, cat. no. AS014) from Abclonal Science, Inc. (Woburn, MA, USA) or Cell Signaling Technology (Danvers, MA, USA). .. All reagents, instruments, and analyzing software for droplet digital polymerase chain reaction (ddPCR), western blotting, ChemiDoc XRS+ system, Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, cat. no. 1610373) were from Bio-Rad Laboratories (Hercules, CA, USA), and NanoDropTM One C was from Thermo Scientific (Waltham, MA, USA). ..

    Article Title: Targeting mitochondrial bioenergetics: the "Achilles' heel" of Leishmania.
    Article Snippet: All chemicals were of analytical grade and obtained from Sigma-Aldrich Chemicals (St Louis, MO, USA), except MTS [3-(4, 5 dimethylthiazol-2-yl) 5- (3-carboxymethoxyphenyl)2-(4-sulphonyl)-2H-tetrazolium] from Promega (Madison, WI, USA), PMS (phenazine methosulphate) from Sisco Research Laboratories (Andheri, Mumbai, India), cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), TRIzol reagent (Ambion, Austin, TX, USA), ThiolTrackerTM Violet, adenosine triphosphatase (ATP) determination kit and MitoSOXTM red [3,8-phenanthridinediamine,5-(6-triphenylphosphoniumhexy l)-5,6dihydro-6-phenyl] (Molecular Probes, Carlsbad, CA, USA), fetal bovine serum (FBS, Gibco) and SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific, (Waltham, MA, USA), Optilux microtest plates for ATP determination and Cell-TakTM (Corning®, Tewksbury, MA, USA), cell lysis buffer and antibodies against AMPKα (AMPactivated protein kinase-α; CST 5831), mTOR (mammalian target of rapamycin; CST 2983), or their phosphorylated counterpart P-AMPKα (CST 2535), P-mTOR (CST 5536), PGC1α (peroxisome proliferator-activated receptor-gamma coactivator; Abclonal A12348), GLUT1 (Glucose transporter 1; Abclonal A6982), β-Actin (CST 4970), HRP-linked anti-rabbit secondary IgG antibody (Abclonal AS014) Cell Signaling Technologies, Danvers, MA, USA) or Abclonal Science, Inc (Woburn, MA, USA) . .. All reagents, instruments, analysing software for droplet digital polymerase chain reaction (ddPCR), western blotting, ChemiDoc AR TIC LE IN PR ES S XRS+ system, Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad 1610373) from Bio-Rad Laboratories (Hercules, CA, USA) and NanoDropTM OneC from ThermoScientific (Waltham, MA, USA). ..

    Digital PCR:

    Article Title: Targeting mitochondrial bioenergetics: the “Achilles’ heel” of Leishmania
    Article Snippet: All chemicals were of analytical grade and obtained from Sigma-Aldrich (St. Louis, MO, USA), except the following: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2 H -tetrazolium (MTS; Promega; Madison, WI, USA); phenazine methosulfate (PMS; Sisco Research Laboratories; Andheri, Mumbai, India); cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA); TRIzol reagent (Ambion, Austin, TX, USA); ThiolTrackerTM Violet, adenosine triphosphatase (ATP) determination kit, and 3,8-phenanthridinediamine,5-(6-triphenylphosphoniumhexyl)-5,6-dihydro-6-phenyl (MitoSOXTM red; Molecular Probes, Carlsbad, CA, USA); fetal bovine serum (FBS, Gibco); SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific, Waltham, MA, USA); Optilux microtest plates for ATP determination and Cell-TakTM (Corning ® , Tewksbury, MA, USA); cell lysis buffer and antibodies against AMPKα (AMP-activated protein kinase-α; CST, cat. no. 5831); mammalian target of rapamycin (mTOR; CST, cat. no. 2983) or their phosphorylated counterpart P-AMPKα (CST, cat. no. 2535); P-mTOR (CST, cat. no. 5536); peroxisome proliferator-activated receptor-gamma coactivator (PGC1α; ABclonal, cat. no. A12348); glucose transporter 1 (GLUT1; ABclonal, cat. no. A6982); β-actin (CST, cat. no. 4970); horseradish peroxidase (HRP)-linked anti-rabbit secondary IgG antibody (ABclonal, cat. no. AS014) from Abclonal Science, Inc. (Woburn, MA, USA) or Cell Signaling Technology (Danvers, MA, USA). .. All reagents, instruments, and analyzing software for droplet digital polymerase chain reaction (ddPCR), western blotting, ChemiDoc XRS+ system, Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, cat. no. 1610373) were from Bio-Rad Laboratories (Hercules, CA, USA), and NanoDropTM One C was from Thermo Scientific (Waltham, MA, USA). ..

    Article Title: Targeting mitochondrial bioenergetics: the "Achilles' heel" of Leishmania.
    Article Snippet: All chemicals were of analytical grade and obtained from Sigma-Aldrich Chemicals (St Louis, MO, USA), except MTS [3-(4, 5 dimethylthiazol-2-yl) 5- (3-carboxymethoxyphenyl)2-(4-sulphonyl)-2H-tetrazolium] from Promega (Madison, WI, USA), PMS (phenazine methosulphate) from Sisco Research Laboratories (Andheri, Mumbai, India), cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), TRIzol reagent (Ambion, Austin, TX, USA), ThiolTrackerTM Violet, adenosine triphosphatase (ATP) determination kit and MitoSOXTM red [3,8-phenanthridinediamine,5-(6-triphenylphosphoniumhexy l)-5,6dihydro-6-phenyl] (Molecular Probes, Carlsbad, CA, USA), fetal bovine serum (FBS, Gibco) and SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific, (Waltham, MA, USA), Optilux microtest plates for ATP determination and Cell-TakTM (Corning®, Tewksbury, MA, USA), cell lysis buffer and antibodies against AMPKα (AMPactivated protein kinase-α; CST 5831), mTOR (mammalian target of rapamycin; CST 2983), or their phosphorylated counterpart P-AMPKα (CST 2535), P-mTOR (CST 5536), PGC1α (peroxisome proliferator-activated receptor-gamma coactivator; Abclonal A12348), GLUT1 (Glucose transporter 1; Abclonal A6982), β-Actin (CST 4970), HRP-linked anti-rabbit secondary IgG antibody (Abclonal AS014) Cell Signaling Technologies, Danvers, MA, USA) or Abclonal Science, Inc (Woburn, MA, USA) . .. All reagents, instruments, analysing software for droplet digital polymerase chain reaction (ddPCR), western blotting, ChemiDoc AR TIC LE IN PR ES S XRS+ system, Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad 1610373) from Bio-Rad Laboratories (Hercules, CA, USA) and NanoDropTM OneC from ThermoScientific (Waltham, MA, USA). ..

    Western Blot:

    Article Title: Targeting mitochondrial bioenergetics: the “Achilles’ heel” of Leishmania
    Article Snippet: All chemicals were of analytical grade and obtained from Sigma-Aldrich (St. Louis, MO, USA), except the following: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2 H -tetrazolium (MTS; Promega; Madison, WI, USA); phenazine methosulfate (PMS; Sisco Research Laboratories; Andheri, Mumbai, India); cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA); TRIzol reagent (Ambion, Austin, TX, USA); ThiolTrackerTM Violet, adenosine triphosphatase (ATP) determination kit, and 3,8-phenanthridinediamine,5-(6-triphenylphosphoniumhexyl)-5,6-dihydro-6-phenyl (MitoSOXTM red; Molecular Probes, Carlsbad, CA, USA); fetal bovine serum (FBS, Gibco); SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific, Waltham, MA, USA); Optilux microtest plates for ATP determination and Cell-TakTM (Corning ® , Tewksbury, MA, USA); cell lysis buffer and antibodies against AMPKα (AMP-activated protein kinase-α; CST, cat. no. 5831); mammalian target of rapamycin (mTOR; CST, cat. no. 2983) or their phosphorylated counterpart P-AMPKα (CST, cat. no. 2535); P-mTOR (CST, cat. no. 5536); peroxisome proliferator-activated receptor-gamma coactivator (PGC1α; ABclonal, cat. no. A12348); glucose transporter 1 (GLUT1; ABclonal, cat. no. A6982); β-actin (CST, cat. no. 4970); horseradish peroxidase (HRP)-linked anti-rabbit secondary IgG antibody (ABclonal, cat. no. AS014) from Abclonal Science, Inc. (Woburn, MA, USA) or Cell Signaling Technology (Danvers, MA, USA). .. All reagents, instruments, and analyzing software for droplet digital polymerase chain reaction (ddPCR), western blotting, ChemiDoc XRS+ system, Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, cat. no. 1610373) were from Bio-Rad Laboratories (Hercules, CA, USA), and NanoDropTM One C was from Thermo Scientific (Waltham, MA, USA). ..

    Article Title: Targeting mitochondrial bioenergetics: the "Achilles' heel" of Leishmania.
    Article Snippet: All chemicals were of analytical grade and obtained from Sigma-Aldrich Chemicals (St Louis, MO, USA), except MTS [3-(4, 5 dimethylthiazol-2-yl) 5- (3-carboxymethoxyphenyl)2-(4-sulphonyl)-2H-tetrazolium] from Promega (Madison, WI, USA), PMS (phenazine methosulphate) from Sisco Research Laboratories (Andheri, Mumbai, India), cDNA Reverse Transcription kit (Applied Biosystems, Grand Island, NY, USA), TRIzol reagent (Ambion, Austin, TX, USA), ThiolTrackerTM Violet, adenosine triphosphatase (ATP) determination kit and MitoSOXTM red [3,8-phenanthridinediamine,5-(6-triphenylphosphoniumhexy l)-5,6dihydro-6-phenyl] (Molecular Probes, Carlsbad, CA, USA), fetal bovine serum (FBS, Gibco) and SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific, (Waltham, MA, USA), Optilux microtest plates for ATP determination and Cell-TakTM (Corning®, Tewksbury, MA, USA), cell lysis buffer and antibodies against AMPKα (AMPactivated protein kinase-α; CST 5831), mTOR (mammalian target of rapamycin; CST 2983), or their phosphorylated counterpart P-AMPKα (CST 2535), P-mTOR (CST 5536), PGC1α (peroxisome proliferator-activated receptor-gamma coactivator; Abclonal A12348), GLUT1 (Glucose transporter 1; Abclonal A6982), β-Actin (CST 4970), HRP-linked anti-rabbit secondary IgG antibody (Abclonal AS014) Cell Signaling Technologies, Danvers, MA, USA) or Abclonal Science, Inc (Woburn, MA, USA) . .. All reagents, instruments, analysing software for droplet digital polymerase chain reaction (ddPCR), western blotting, ChemiDoc AR TIC LE IN PR ES S XRS+ system, Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad 1610373) from Bio-Rad Laboratories (Hercules, CA, USA) and NanoDropTM OneC from ThermoScientific (Waltham, MA, USA). ..

    Blocking Assay:

    Article Title: Prenatal Exposure to a Moderate Dose of Δ9-Tetrahydrocannabinol Alters Hippocampal AMPA Receptor Channel Function Without Changing Subunit Expression
    Article Snippet: .. Following blocking, primary antibodies raised in different host species were then selected and applied simultaneously as a multiplex cocktail, with targets chosen to have distinct apparent molecular weights for overnight incubation at the same time, according to the molecular weights of the target proteins, as determined by Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, Benicia, CA, USA), allowing multiple proteins to be probed on the same membrane while minimizing antibody use. ..

    Multiplex Assay:

    Article Title: Prenatal Exposure to a Moderate Dose of Δ9-Tetrahydrocannabinol Alters Hippocampal AMPA Receptor Channel Function Without Changing Subunit Expression
    Article Snippet: .. Following blocking, primary antibodies raised in different host species were then selected and applied simultaneously as a multiplex cocktail, with targets chosen to have distinct apparent molecular weights for overnight incubation at the same time, according to the molecular weights of the target proteins, as determined by Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, Benicia, CA, USA), allowing multiple proteins to be probed on the same membrane while minimizing antibody use. ..

    Incubation:

    Article Title: Prenatal Exposure to a Moderate Dose of Δ9-Tetrahydrocannabinol Alters Hippocampal AMPA Receptor Channel Function Without Changing Subunit Expression
    Article Snippet: .. Following blocking, primary antibodies raised in different host species were then selected and applied simultaneously as a multiplex cocktail, with targets chosen to have distinct apparent molecular weights for overnight incubation at the same time, according to the molecular weights of the target proteins, as determined by Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, Benicia, CA, USA), allowing multiple proteins to be probed on the same membrane while minimizing antibody use. ..

    Membrane:

    Article Title: Prenatal Exposure to a Moderate Dose of Δ9-Tetrahydrocannabinol Alters Hippocampal AMPA Receptor Channel Function Without Changing Subunit Expression
    Article Snippet: .. Following blocking, primary antibodies raised in different host species were then selected and applied simultaneously as a multiplex cocktail, with targets chosen to have distinct apparent molecular weights for overnight incubation at the same time, according to the molecular weights of the target proteins, as determined by Precision Plus ProteinTM All Blue Prestained Protein Standards (Bio-Rad, Benicia, CA, USA), allowing multiple proteins to be probed on the same membrane while minimizing antibody use. ..



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